BxGenomics enables biologists to easily analyze RNA-Seq data, identify changed genes and enriched pathways, and further visualize the results and compare across projects using interactive data mining tools.
Biologically Meaningful Answers
Immediately find changed genes and functional pathways in instant reports.
Full Potential of Data
Explore your results in an interactive online database to delve into details.
Expert Bioinformatics Support
Rest assured that your data are in good hands and help is always available.
BxGenomics is an interactive web-based platform that provides user-friendly tools to analyze and explore RNA-seq data. BxGenomics enables biologists to easily analyze RNA-Seq data, identify changed genes and enriched pathways, and further visualize the results and compare across projects using interactive data mining tools.
Tip: Hover or click the text in above diagram to view examples.
Upload your raw RNA-Seq data, and use the automatic pipeline in BxGenomics to perform RNA-Seq data analysis and receive easy-to-understand reports.
|Fast and Easy Service|
RNA-Seq Analysis service is easy to use. Just provide raw data and sample description, and a professional report and personal online database will be delivered to you in 1-2 weeks.
An expert-designed analysis pipeline will work for you behind the scenes, including comprehensive QC of raw data and gene counts, robust statistical analysis for differentially expressed genes, advanced functional pathway analysis, and more. All results are reviewed by experts before final delivery.
|Meaningful and Easy-to-Understand Report|
The reports are designed to be easily understandable by biologists. It is based on experience working with hundreds of researchers on RNA-Seq projects. The easy-to-read reports place the most relevant biological information on the front page, with technical details in secondary links.
|Differentially Expressed Genes (DEGs)|
Robust methods are used to identify DEGs, to identify what has changed between biological conditions. Results can be viewed as a summary table, individual gene lists, or a heatmap. Results can also be exported to Excel.
|Enriched Pathways from Changed Genes|
BxRNASeq provides functional enrichment results from two methods, hypergeometric test and gene set enrichment analysis (GESA).
The hypergeometric test compares DEGs with all genes in the genome to find enriched or over-represented functions.
GESA examines the fold change of all genes and focuses on changes of expression of groups of genes within the same biological pathway. These two methods often provide similar and complimentary reports.
|Comprehensive Quality Assessment|
It is important to avoid garbage in, garbage out when processing genomic data. BioInfoRx's RNA-Seq Analysis Service incorporates several QC steps to ensure reliable and accurate results.
Raw data QC ensures the sequencing run generates high quality data, gene level QC detects issues like PCR duplications or contaminated samples, finally sample grouping is critical to verify biological differences are above technical variations in the experiment.
|Access Results Easily Online|
Access your results on a secure website, including all the raw data, alignments, QC, DEG and functional enrichment results. Scientists can directly load the read alignments from our server to genome browsers. Full description of methods is included which can be used in manuscripts.
|Gain Biological Insights Through Data Visualization|
A picture is worth a thousand words, and BxGenomics data mining platform helps you create meaningful graphs from your data easily.
|Achieve Data Integration and Archive in One Step|
Once the RNA-Seq data is upload to the BxGenomics data mining platform, authorized users can access the data with a browser anytime from anywhere with internet connection.
Different kinds of gene IDs are automatically recognized and converted so gene expression data are easily integrated between different projects analyzed with different types of gene IDs.
The BxGenomics platform serves dual purposes, as a data mining system of all RNA-Seq results in the laboratory to enable continuous discovery, and as a data archive system to enable data longevity and long-term access.
|Gene Expression Graphs|
View one or a few genes using gene expression tools, or use heatmap to look at the profiles of many genes.
The interactive PCA tools gives a quick and easy way to view sample grouping and decide on the most important differences to focus on.
The heatmap tool can also cluster samples to give further insights on sample grouping and how specific genes contribute to the grouping between focuses on changes of expression of groups of genes within the same biological pathway.
These two methods often provide similar and complimentary reports.
To view comparison data, or gene expression changes between samples, volcano plots gives a nice overview, and bubble plots can help users focus on specific gene groups.
|Enriched Functions and Pathway|
For any uploaded comparisons, BxGenomics system runs a comprehensive evaluation of enriched pathways and provides summary graphs as well as detailed reports. The pathway heatmap tool can display enriched pathways across several related comparisons.
Map gene changes to your favorite pathways and delve into the details of gene regulation of specific biological functions. BxGenomics supports pathway overlap for KEGG pathways, Wikipathways, and Reactome Pathways.
|Knowledgeable and Helpful Service Team|
The BxGenomics RNA-Seq service team consists of highly educated scientists and customer support managers with years of experience in biological studies and genomics.
The team works collaboratively with customers to provide the utmost service for the success of every RNA-Seq project. Customers benefit from continuous high-quality technical support by a friendly and responsive team.
|Accessible Demo Data and Detailed User Guides|
New users can use demo data curated by our team to test the system, and get familiar with the RNA-Seq pipeline and various other features. The data mining system also comes with a lot of public data ready to be explored and visualized. Scientist can easily compare their own data with the pre-loaded public data. Our support team have also produced numerous help document and user guides to help you go through each step.